Curcumin longa, a nutritional supplement is a rich source of polyphenolic curcuminoids: curcumin, demthoxycurcumin and bisdemethoxycurcumin. it has potent antioxidant, anti-iinflammatory, and anti-tumour activities. A recent report (Phytochem. Anal. 2009, 20, 372-377) described the analysis of curcumin metabolites by direct analysis in real time mass spectrometry (DART-MS) directly from TLC plates exhibiting molecular ion peaks [M+H]+ M/Z 309, 339 and 369 respectively. We have extended our observations and have analyzed four curcumin metabolites using argentation TLC plates prepared in our laboratory. Using solvent system CHCl3: CH3OH 18:2 (v/v) inidividual compounds were separated and analyzed via preparatory AgNO3-TLC SiO2 gel paper plates with aluminum backing. All four compounds including the dihydrosaturated derivative of curcumin were visualized under UV light. The present studies will describe in detail the anti-glycosylation actitivity of all the curcumin metabolites and their comparison with our ongoing anti-diabetic studies with Okra seed extract.

Separation of curcuminoids in turmeric by argentation thin-layer chromatography and their comparative antidiabetic activity with okra seed extract

248th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.