Foliar uptake and phloem transport of N-[3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl)sulfinyl]-1H-pyrazol-5-yl]-1-(�-D-glucopyranosyl)-1H-1,2,3-triazole-4-methanamine (GTF), a glucose-fipronil conjugate, involves a carrier-mediated mechanism in castor bean seedlings. In this study, root uptake and long-distance root-to-shoot transport of GTF in soybeans were characterized. GTF exhibited a distribution pattern that differed from fipronil via root uptake in soybean seedlings. The soybean plants were transformed using RcHEX3, a monosaccharide transporter. The long-distance root-to-shoot transport of GTF in the transgenic soybean composite plants indicated that RcHEX3 increased the root uptake and the xylem loading of GTF. A conjugate of GTF with a fluorescent moiety (FPGN) was used to visualize the root uptake and the transport pathway of GTF by confocal laser scanning microscopy. FPGN was mainly visible in the xylem and the pericycle in the sterile hairy roots of soybeans. These results suggested that the long-distance root-to-shoot translocation of GTF also involved a carrier-mediated mechanism. It may be possible to synthesize new pesticides which can be transported by carriers to sink tissues where need protection most.

Root uptake and long-distance root-shoot translocation of a glucose-fipronil conjugate in soybeans (<i>Glycine max</i>)

248th National Meeting (2014)

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Root uptake and long-distance root-shoot translocation of a glucose-fipronil conjugate in soybeans (Glycine max)