RNA interference (RNAi) is a process in which messenger RNA (mRNA) is destroyed, silencing the corresponding genes. This process is used to study and regulate gene expression. Occasionally, genes other than the target are silenced. This research examines the scientific principles that govern transitive RNA interference and off-target effects by targeting a sequence 3’ away from the hairpin (37a), 5’ from hairpin (37b) and the hairpin sequence itself (37hp). These plasmids were fed to C. elegans by E. coli to test for off-target effects. RNA expression levels were determined using qPCR. It was found that dsRNAi is effective in knocking out the desired genes but also causes off-target effects.

Evaluation of off-target effects through double-stranded RNA interference in C.elegans

249th National Meeting (2015)

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