Immune activation and the effectiveness of vaccine adjuvants are modulated by the detection of specific molecular activators via pattern recognition receptors (PRRs). The cytosolic PRR nucleotide-binding and oligomerization domain 1 (NOD1) has been shown to play distinct roles in both inflammation and vaccine adjuvants. Despite the implications of NOD1 in disease, the spatial and temporal aspects of the molecular activators of NOD1 have not been determined. Here, we present remote activation of NOD1 via photocaged NOD1 agonist. Based on established SAR for the molecular activator of NOD1, D-glutamyl-<i>meso</i>-diaminopimelic acid (iE-DAP), we have strategically protected the agonist with a photolabile protecting group, 6-nitroveratryloxycarbonyl carbamate (NVOC). With this photocaged agonist, NOD1 activity is suppressed and then regained upon exposure to light. We confirmed NF-κB production upon exposure to light in a model cell line, as well as activity in primary cells by examining cytokine production in bone marrow derived dendritic cells (BMDCs). This will allow us to better explore the spatial and temporal mechanism of NOD1 activation, leading to a better understanding of immune signaling.

Light controlled activation of immune cells via photocaged NOD1 agonist

249th National Meeting (2015)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.