Purification of viruses can take advantage of their large size compared to most contaminants of interest. A novel chromatographic flow through mode technique for virus purification is supported by media such as Capto Core 700<sup>TM</sup>, which contains an inert size-selective shell surface with an exclusion limit of 700 kDa with a multimodal octylamine ligand core. In this work we have adapted this technology to virus detection, and demonstrated the method using M13 phage as a model virus. We added enzyme-labeled target-specific antibodies to samples with/without M13, and separated them on a Capto column. In the absence of M13 target, the antibody-enzyme conjugate is small enough to enter the core and be adsorbed to undetectable levels. In the presence of M13, antibody-enzyme conjugate can bind to the virus and be excluded from adsorption, and detected in the flow-through.

Virus detection using restricted-access adsorbents

249th National Meeting (2015)

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