Endotoxins are chemically lipopolysaccharides (LPS) and their presence in food or environment is an indication of bacterial contamination. They are the major component of the outer-membrane of the Gram-negative bacteria and also responsible for their toxic effects. A novel method based on electrochemical impedance spectroscopy and using Toll-like receptor 4 (TLR4)/myeloid differentiation-2 (MD-2) complex as the bio-recognition element was developed to detect endotoxins in environmental waters. This was achieved by modification of gold sensors with TLR4/MD-2 through lipoic acid N-hydroxysuccinimide ester. A linear relation between the LPS concentration and the change in Rct (impedance data) was observed which could be used to quantify endotoxins. This method provides a platform for fast, convenient and cost-efficient detection of bacteria in water.

Development of an impedance-based TLR4/MD-2 complex-modified Au biosensor for detection of endotoxins from Gram-negative bacteria

249th National Meeting (2015)

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