The Serpin complexes that help to regulate the melanization cascades in the <i>Anopheles gambiae</i> mosquito are not very well understood but are important due to their connection to the regulation of the mosquitos’ immune system. The complexes in which serpin 2 participates as a binding partner were initially separated by magnetic bead immunoaffinity chromatography using polyclonal antibodies to serpin 2. The eluted fraction from this experiment was subjected to trypsin digest and then analyzed by LC/MS. Peptides and proteins in the sample were identified using SEQUEST® and MASCOT®. Several possible binding partners to Serpin 2 were tentatively determined. Biological studies are currently underway to confirm the binding interactions.

Identification of Serpin 2 complex binding partners in <i>Anopheles gambiae</i> by immunoaffinity chromatography and mass spectrometry

249th National Meeting (2015)

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Identification of Serpin 2 complex binding partners in Anopheles gambiae by immunoaffinity chromatography and mass spectrometry