Previous work has shown that ferrocene tethered to the nitrogen of linear polyethylenimine (Fc-Cn-LPEI) is an effective electron mediator for chemically crosslinked, solution cast glucose bioanodes. Layer-by-layer assembly of redox polymer/enzyme films provides a fast and efficient means to decrease the fabrication processing time. Films constructed from Fc-Cn-LPEI and periodate modified glucose oxidase (p-GOX) were characterized by varying the mediator tether length (three or six carbons) and the washing time between polymer/enzyme layers. The physical processes by which films are fabricated was elucidated by looking at the change in the electrochemical and steady-state enzymatic response of films assembled with various numbers of bilayers. Once the tether length and wash time were selected, Fc-Cn-LPEI/p-GOX films were used as the anode in a glucose/O2 biofuel cells using a commercially available air-breathing platinum cathode. The electrochemical approximation of film thickness, distribution of ferrocene throughout the films, and the effect of pH on the biofuel cell will all be discussed.

Development and characterization of layer-by-layer assembled ferrocene polymer/enzyme films for usage as biofuel cell anodes

249th National Meeting (2015)

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