Advancements in mammalian cell culture techniques are fostering a landscape shift on upstream production processes in the biopharmaceutical industry. Tangential flow filtration (TFF) perfusion assemblies allow for a continuous separation of biomass from the protein of interest when employing a microfiltration membrane. In addition to a higher productivity process, the combination of the primary recovery and cell culture steps results in an alignment for continuous purification processes. In this study, the influence of membrane’s chemistry, structure and pore size on the antibody’s sieving coefficient is investigated, where several single-use hollow fiber TFF membranes are operated independently during a single Chinese Hamster Ovary (CHO) perfusion cell culture process. The results of this study are not only significant for evaluating the membrane characteristic’s impact on the target protein sieving, but also to increase the understanding of the fouling event in such complex systems.

Antibody sieving analysis of multiple microfiltration membranes during a single perfusion cell culture

249th National Meeting (2015)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.