The currently accepted mode of operation for enzymatic saccharification reaction is batch, although sequential batch and multiple-CSTR modes have been proposed and tested at pilot scales. Fed-batch and continuous modes for enzymatic hydrolysis in one reaction vessel, with retention of the undigested solid biomass, have been proposed in the literature. However, there are several technical challenges to be overcome before the proposed concepts could be employed to enable continuous enzymatic saccharification at pilot and commercial scales. This work presents initial studies of continuous membrane fractionation of sugars, enzymes, polysaccharides, and lignin fractions using commercial membranes. Results include measurements made with model mixtures, as well as, integrated studies employing a CSTR, fed with acid hydrolyzed, corn stover-derived lignocellulosic biomass, and continuous membrane fractionation of produced sugars. The data is interpreted in the context of a simple reactor design model, which can be probed to identify targets for improved technology.

Continuous enzymatic hydrolysis: Membrane fractionation

249th National Meeting (2015)

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