Today’s macromolecules, including synthetic and natural/biological polymers are highly fine-tuned to exhibit their best properties. The molar mass, the chemical composition, the architecture and other parameters are optimized for robust structure-property function relationships.<br/>Size-exclusion chromatography (GPC, SEC, GFC) is often applied to characterize macromolecular species. Since GPC/SEC/GFC separation is not governed by molar mass but hydrodynamic volume, copolymers, polymer architectures and complex macromolecular formulations cannot be characterized easily.<br/>However, the combination of different chromatographic separation methods (HPLC, GPEC, SEC, TGIC, SFC, CE, TREF, etc.) into a 2-dimensional (2D) setup can lead to significantly enhanced information content.. Accurate and precise results can be directly derived for each sample component in only one 2D run, e.g. molar mass, chemical composition, endgroup, functionality, degree of branching, architecture, aggregation, etc, depending on the specificity of the selected separation techniques.<br/>This presentation will give application examples from various hyphenations and discuss how the on-line setup can be optimized with respect to easy-to-use and fully automated transfer from the first to the second dimension. Options for data presentation using different plot and evaluation types and access to the final results will also be shown.

Use of 2-D chromatographic techniques to elucidate macromolecular structures

249th National Meeting (2015)

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