In single cell analysis, developing high throughput microfluidic devices is essential for fast and efficient analysis. Many fluid handling techniques allows transporting 25-40 cells/h. Traditional fluid handling techniques such as vacuum/pressure driven flow always suffering with low through puts. In these techniques, flow rates are less controllable and small adjustments cannot be made. We introduced novel fluid handling technique in order to increase the analysis rate up to ~1000 cells/h. On-chip integration of miniaturized peristaltic pumps are used  to control the fluid flow throughout the microfluidic channel network. Set of micro pumps were confined in to small device area and they were pneumatically actuated in order to utilize them as peristaltic pumps. These pumps are simple, low cost, durable, easy to fabricate and bio-compatible. They provide precise control of the flow rate, and produce continuous flows up to 9.2nL/s. Two-layer microfluidic chip was fabricated using multilayer soft lithographic technique. Jurkat cells were labeled with 6-carboxyfluirescein(6-CFDA) and Oregon green (OG). Peristaltic pumps were used to drive the cells automatically from sample reservoir to waste reservoirs. Cells were lysed and electrophoretically separated using same electric field.  Two fluorescent dyes were detected using laser induced fluorescent(LIF) detection system.

Designing and characterization of high throughput multilayer micro fluidic device for single cell analysis

249th National Meeting (2015)

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