A highly sensitive platform was developed to determine anionic polar metabolites by coupling capillary ion chromatography with high resolution accurate mass spectrometry. This method provided separations of isobaric sugar monophosphates, sugar diphosphates, and organic acids using a capillary format anion-exchange column optimized for high resolution and efficient separations of organic acids using supermacroporous, 4-µm particle resin particles. The analytes were detected by an orbitrap mass spectrometer resulting in mass accuracy &lt; 1 ppm and fmole on column sensitivity.<br/><br/>This method was applied to metabolomic profiling of metabolic biomarkers for oral squamous cell carcinoma (OSCC) metastasis, 3 paired OSCC cell lines (UM1, UM5, CSC) with wild-type controls. Cap IC demonstrated outstanding separation and peak shape for anionic polar metabolites with increased sensitivities &gt;100-fold as compared to RPLC and HILIC methods. Differential analysis revealed significant changes in energy metabolism pathways, e.g., glycolysis cycle and tricarboxylic acid cycle.

Profiling anionic polar metabolites in oral cancer using capillary ion chromatography and high resolution accurate mass spectrometry

249th National Meeting (2015)

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