Hameed S., Jurica J.<br/>Differential Remnant Lipoprotein Profiling via Immunoseparation and Dynamic Light Scattering Analysis<br/>Remnant lipoprotein (RLPs), products of partially catabolized chylomicrons or very low density lipoproteins, are strongly correlated with an increased risk of cardiovascular complications such as atherosclerosis. Remnant lipoproteins of VLDL and chylomicrons, along with these particles comprise the triglyceride rich lipoprotein (TRL) subclass, which are responsible for the transport of dietary fats in human blood. Although there are methods to quantify RLP cholesterol, no known method exists by which these RLPs and their pre-catabolized TRL versions can be analyzed by size. By examining the size distribution of the remnant lipoprotein subclass and the entire TRL subclass, one can obtain a differential analysis of an individual’s metabolism in regard to the formation of these particles. The goal of this research is to isolate the remnant lipoprotein subclass along with the nascent TRL particles in order to determine the size distribution of the entire subclass via dynamic light scattering (DLS). This information is used to quantify the relative numbers of each member particle in the TRL subclass as a clinical evaluation tool. The RLPs are separated by an immunoseparation assay containing antibodies to the apo B-100 and apo A-1 proteins that are found on all other major lipoprotein subclasses. The RLP particles are not separated by these antibodies and can then be characterized by size utilizing DLS. The TRL subclass is isolated in its entirety via a sequential double spin high-speed centrifugal flotation and is also characterized by DLS. The RLP size distribution is overlaid on the entire TRL subclass distribution as a differential analysis. This unique examination provides information to assess the features involved in an individual’s metabolism and atherogenic risk status and may prove to be a clinically viable diagnostic tool.

Differential remnant lipoprotein profiling via immunoseparation and dynamic light scattering analysis

249th National Meeting (2015)

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