Hybrid nanomaterials which incorporate inorganic photo-active nanoparticles (NPs) and enzymes immobilized on different supports have recently captured the attention of many scientists and technologists. These systems have been extensively used on a large number of applications including biomedical and environmental analytic and diagnosis tools, photocatalysis, and photodynamic cancer therapy, etc. For instance, it has been reported that semiconductor NPs, as well as gold NPs, are able to generate reactive oxygen species upon illumination with light with the appropriate wavelength that can be used as co-substrate in numerous peroxidase catalyzed reactions, having in this way a photo-active catalytic system. On the other hand, cellulose nano- and microfibers have been used as supports to immobilize the aforementioned hybrid composite systems. Cellulose have several interesting properties such as high strength and stiffness, ease of functionalization, low weight and cost, biodegradability and renewability, which make it a first order candidate material to be used as support for the immobilization of NPs. Self-assembling processes based on electrostatic, hydrophobic, hydrogen bonds, or through conjugation with specific ligands might be an efficient strategy to produce novel composite materials combining the optical useful properties of inorganic NPs with the structural virtues of cellulose fibers. Therefore, the study of photo-active NP-cellulose interfacial interactions as well as the stability of the conjugated systems is of great interest for the development of immobilized hybrid nanomaterials. With this aim, in this work we will present Quartz Crystal Microbalance adsorption study of the immobilization of NP-enzyme complexes, composed by peroxidases and mercaptoacetic acid-capped CdS NPs or citrate-capped gold NPs, at surfaces modified with cellulose obtained from algae residues and “nejayote” (a liquid mixture produced as a residue of corn tortilla production). In the latter case, cellulose obtained was functionalized with ferulic acid trough a laccase enzymatic treatment. FTIR, and UV vis spectra confirmed the covalent bond between cellulose and oxidized ferulic acid. Adsorption isotherms at different temperature, pH and electrolyte conditions were considered to evaluate the NP-enzyme-cellulose interfacial interactions.

Adsorption of inorganic photo-active nanoparticle/enzyme hybrid systems on surfaces modified with cellulose obtained from natural and industrial residues: A QCM study

249th National Meeting (2015)

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