Production of chemicals of industrial value from renewable materials is an appealing means to reduce the organic, inorganic and greenhouse gas waste streams associated with the synthesis of many of these compounds from petroleum distillates. β-carboxy-cis,cis-muconic acid and 3-carboxymuconolactone represent two novel polymer precursors which are readily formed from D-glucose (via the shikimate pathway) through heterologous biosynthesis. The target triacid and lactone are both biosynthesized by an E. coli host containing a multi-monocistronic construct comprised of genes from siderophore biosynthesis (dehydroshikimate dehydratase) and aromatic compound catabolism (dihydroxybenzoate dioxygenase). In this work, we have studied the effects of temperature and pH on the productivity of a strain expressing this synthetic construct. We have also investigated the effect on productivity of replacement of the native dehydroshikimate dehydratase with a mutant enzyme which has been computationally designed for an increased thermal resistance.

Influence of the role of dehydroshikimate dehydrogenase in production of β-carboxymuconic acid from D-glucose using an Escherichia coli heterologous host

249th National Meeting (2015)

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