We developed a nanometric gap structure between substrate-supported model membrane and silicone elastomer (polydimetylsiloxan; PDMS) as a novel biosensing platform. A micropatterned membrane comprising polymeric and fluid lipid bilayers was generated on a glass substrate by the lithographic polymerization of diacetylene phosholipid and subsequent incorporation of natural (fluid) lipid bilayers. We chemically functionalized the surface of the polymeric bilayer with biotin moiety and bonded a flat PDMS slab onto it by using the biotin-streptavidin linkage. We employed lipid vesicles having biotin moiety as a bonding agent between the polymeric bilayer and PDMS. Due to the finite size of lipid vesicles, a very thin gap was formed between the fluid lipid bilayer and PDMS. The thickness of the gap was estimated to be less than 100 nm. Proteins that could bind onto the fluid bilayer by molecular recognition was selectively transported into the gap structure by the lateral diffusion within the bilayer. The combination of nanogap structure and selective transport by the lipid bilayer offers a novel platform for selective and sensitive detection of biological molecules.

Nanometric gap structure between substrate-supported model membrane and silicone elastomer

249th National Meeting (2015)

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