Cannabis sativa is a herbaceous plant that may be cultivated for various uses. This uses include the production of oil and fiber as well as recreational usage. Active compounds in cannabis sativa include tetrahydrocannibol, THC, and over 60 additional cannabinoids, notably cannabidiol and cannabinol. THC is metabolized to 11-hydroxy-THC, which is subsequently metabolized to 11-nor-carboxy THC. This metabolite is further conjugated to aid in renal excretion.  In a forensic toxicology laboratory, THC and its metabolites may be analyzed in various sample matrices, such as urine, oral fluid and blood. Detection is dependent upon the sample matrix, specimen preparation and analytical methodology used.  In this presentation, sample preparation for urine, oral fluid, and blood will be described. In addition, analytical methodologies such as gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry/mass spectrometry will be discussed. Lastly, representative data will be reviewed. Furthermore, real-case examples will be used to demonstrate how fundamental chemical principles impact testing methodologies as well as the roles of chemists in analysis of donor specimens. Additionally, criteria for successful transition from college to the workforce will be discussed, including current position requirements and employment opportunities at a high complex forensic toxicology laboratory.

Forensic toxicology of marijuana

249th National Meeting (2015)

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