Homodecoupled band-selective (HOBS) NMR methodology was tested to see how it could enhance the resolution of sugar (cellobiose octaacetate) and nucleic acid (6-, 10-, and 16-mer oligonucleotides) spectra. Using the basic HOBS acquisition sequence for one-dimensional proton experiments, pulse programs were generated and tested for advanced NOESY, ROESY, and TOCSY experiments. The efficacy of HOBS acquisitions was compared to those of both normal and purely band-selective pulse programs in both one- and two-dimensional methods. With the 6-mer, direct comparisons were made between measurements of T1 relaxation delays in both HOBS-style and normal acquisitions. Using HOBS methods, values obtained for T1 were more precise than those measured under a standard method due to the enhanced resolution brought forth by decoupling – in the H5 region of the spectra, the standard deviations of T1-values determined by peak-picking decreased by as much as 0.033 s when implementing HOBS techniques. Finally, HOBS methods were compared to two other pure-shift related methods – instantaneous pure shift (IPS) and pure shift yielded by chirp excitation (PSYCHE).

Investigation of HOBS methodology in nucleic acid NMR studies

249th National Meeting (2015)

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