The open–closed equilibrium of xanthene dyes like rhodamine B is a key characteristic of fluorescence to understand and compare in novel probes. Rhodamine B and related compounds are pH insensitive over a significant range, making comparison difficult. However, the local environment, including the polarity of the solvent, can affect the equilibrium between the closed, nonfluorescent state (<b>2</b>) and the open, fluorescent state (<b>1</b>). We have developed an efficient polarity assay to characterize and compare structurally unique rhodamine B derivatives and have optimized the assay for more general use to investigate novel probes.

Designing an efficient and practical polarity assay for xanthene dyes

249th National Meeting (2015)

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