Burkholderia cenocepacia is a gram-negative bacteria that is a part of the Burkholderia cepacia complex. These bacteria are opportunistic pathogens, found most commonly in cystic fibrosis patients. One of the hallmarks of these bacteria is their broad antibiotic resistance due in part to the unique structure of the outer membrane of the cells. To understand these resistance mechanisms, a tool to perform gene replacement studies is being constructed. Beginning with the suicide vector pEX100T, a B. cenocepacia specific resistance cassette was added. The gene of interest is BCAL0894, a potential B. cenocepacia membrane protein.

Construction of a Burkholderia cenocepacia-specific gene replacement vector

249th National Meeting (2015)

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