The ability of humic acid to bind to pollutants is important to determine their transport and bioavailability. Static quenching was observed by fluorescence and UV-Visible spectroscopy at 25, 30, 35, 40, and 45⁰C. Stern-Volmer plots allowed for determination of the equilibrium binding constants for a variety of temperatures that were averaged to construct a van’t Hoff plot. The enthalpy of binding between acridine yellow G (AYG) and humic acid and norfloxacin and humic acid were determined from van’t hoff plots to be -15.4±7.9 and -10.3±3.8 kJ/mol. Since the magnitude of the enthalpy of binding is greater for AYG than norfloxacin, AYG will more readily join with soil and will have a lower level of bioavailability.

Static quenching of acridine yellow G and norfloxacin by humic acid

249th National Meeting (2015)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.