Students often fail to see the connection between the Organic Chemistry and Biochemistry. We worked with a research student on a combined lab experiment. The research project proposed examines the synthesis of two known inhibitors, difluorosuccinic acid and 2,4-dinitrophenol, and their effect on succinate dehydrogenase. The question posed is how the two synthesized inhibitors will obstruct the succinate dehydrogenase’s enzyme ability. To assess the type and the effectiveness of these inhibitors on succinate dehydrogenase in isolated yeast cells, we will utilize the knowledge of enzyme kinetics in conjunction with Succinate Dehydrogenase Activity Colorimetric Assay Kit. This proposed lab will enhance the laboratory skill for the Meredith students since so many of our organic students also take Biochemistry I. We proposed that the lab can be done in two periods in both Organic Chemistry I and Biochemistry I Labs and will give our students a real application of synthetic experience and enzyme inhibition.

Connecting organic chemistry and biochemistry

249th National Meeting (2015)

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