Chlorogenic acid (CGA) is a secondary plant metabolite with chemotherapeutic and chemopreventative potential along with other purported health benefits. The primary objective of this study was to use microwave-assisted extraction (MAE), which was optimized in<br/>a previous study, to quantify and compare concentrations of CGA and caffeic acid (CA), a product of the decomposition of CGA, in the skins and flesh of various fruits, and ultimately to compare concentrations in organically and conventionally grown fruits. High performance liquid chromatography (HPLC) with a multi-wavelength detector was used for analysis. A spike recovery study was performed for tomatoes and peaches/nectarines, and high recoveries were achieved from MAE extraction. The third objective of this study was to optimize a dispersed liquid-liquid microextraction (DLLME) technique for extracting pesticides from fruit skins to determine if there is a correlation between pesticide levels and CGA and CA concentrations. Pesticides were analyzed by gas chromatography-mass spectrometry (GC/MS) using a selected ion monitoring method.

Determination of chlorogenic acid and caffeic acid in fruits with evaluation of pesticide concentrations

249th National Meeting (2015)

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