The fundamental of nature is structure determines function; as such it is natural for biological molecules (biomolecules) with active functions to be dependent on structural characteristics such as chirality and anisotropy. Therefore, one field of research is to develop robust, sensitive, specific techniques capable of determining biomolecules’ proper structural characteristics and to a greater extent to be able to detect distinct molecules. A complete study of organic ligand Tetracycline (TC) chelated with luminescent Europium(III) (Eu) associated with biological molecules in relation to pH and concentration was conducted using steady-state luminescence, time-resolved luminescence, and 5D0←7F0 europium excitation spectroscopy. Furthermore, we utilized circularly polarized luminescence (CPL) to monitor the change in chirality in relation to the TC-Eu complexes association with biological molecules. The resulted spectra exhibited explicit peaks of each TC-Eu species associated with biological molecules at various pH and concentration in time-resolved and steady-state luminescence that can be used as a “fingerprint” to (1) be used to identify unknown molecules similar to the usage of molecules’ distinct vibration modes in IR and NMR techniques to determine the unknown molecules’ structures. Also, CPL spectra of the TC-Eu probe associated with targeted molecules can be used to (2) give a more extensive view on chirality of targeted molecules.

Alternative to detection: Europium(III)-tetracycline species association with biological molecules

249th National Meeting (2015)

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