Formation of antibiotic-resistant bacterial biofilms on indwelling medical devices poses a critical problem in healthcare settings, being responsible for the majority of hospital-acquired infections. Despite the recent advances in antimicrobial research, efficient antibiofilm strategies to control the adhesion and proliferation of bacteria on medical devices are still needed. Herein, hybrid coatings comprising acylase and α-amylase built on silicone urinary catheters using a layer-by-layer technique were investigated as alternatives to the conventional antibiotic treatments for prevention of bacterial biofilm formation and further emergence of resistance. The assembling of multilayer coatings on the catheter surface was achieved by alternate deposition of negatively charged enzymes and positively charged polyethylenimine. These hybrid enzyme coatings were able to quench quorum sensing process and degrade biofilm adhesive components, thereby synergistically inhibiting biofilm formation by Pseudomonas aeruginosa. Furthermore, the dual-species biofilm occurrence (Pseudomonas aeruginosa and Escherichia coli) on urinary catheter was significantly reduced under dynamic conditions in an in vitro catheterized bladder model, when compared to the non-modified specimen. These antibiofilm active coatings that integrate anti-quorum sensing and anti-adhesive strategies did not affect the viability of the human fibroblasts (BJ-5ta) over seven days and could be a viable alternative to control pathogenic bacterial biofilms on indwelling medical devices.

Enzyme multilayer coatings prevent bacterial biofilm formation on urinary catheters

249th National Meeting (2015)

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