The recalcitrance nature of biomass is one of the main obstacles in biorefineries operating under the bioconversion platform to convert cellulose to sugars and other chemical species. The presence of lignin hinders the enzymatic hydrolysis of lignocellulosic materials, through hydrophobic and electrostatic interactions. This study pertains a fundamental study of these interactions. To this end, lignins from different wood sources (Hardwood and Softwood) were isolated by the Milled Wood Lignin (MWL) method. Compositional spectroscopic analyses of the MWL was carried out and lignin films were prepared by the spin coating technique. The films were characterized by atomic force microscopy and contact angle measurements. The interactions of cellulase enzymes with the different lignins where monitored with Quarz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) and the main conclusions are presented.

Mill wood lignin interactions with monocomponent cellulases

249th National Meeting (2015)

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