Herein we studied the exploitation of carbohydrate polymers to prepare a poly interface on cellulose paper for biomolecule conjugation. As a potential platform for point-of-care clinical analyses and environmental diagnostics, cellulose paper-based bioanalytical devices have sparked growing attention. Until now, different strategies to immobilize biomolecules onto paper support have been studied. In this work, we demonstrate the functionalization of the cellulose paper surface with two typical carbohydrate polymers (i.e., chitosan and sodium alginate) respectively, which enables cellulose paper to bear commonly used functional groups for bioassays. Then in order to improve the understanding about the morphology and immobilized biomolecule performance on polysaccharide modified paper biosensing platform, protein immobilization studies were performed. The interfaces between biomolecules and modified cellulose fibres via different methods were characterized by fluorescence microscope and scanning electron microscopy. The developed polysaccharide-coating chemistry is expected to open new approaches for using functionalized cellulose in bioassays like immunodiagnostic applications.

Carbohydrate polymer-coating chemistry for cellulose based bioassays

249th National Meeting (2015)

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