Age-related macular degeneration (AMD) is the leading cause of irreversible visual impairment in the developed world. The precise, rapid detecting and monitoring of vascular endothelial growth factor (VEGF) are critical for improving anti-VEGF clinical treatments of AMD. An impedance biosensor based on poly(3,4-ethylenedioxythiophene) (PEDOT) carboxylic acid/PEDOT copolymer was developed as a promising technique for detecting VEGF. Carboxylic acid modified 3,4-ethylenedioxythiophene (EDOT)/EDOT was electrochemically polymerized on the surface of an interdigitated gold electrode. The thin nanopolymer films of PEDOT carboxylic acid/PEDOT were characterized using optical microscopy, SEM/EDS and FIB techniques. Anti-VEGF monoclonal antibody from Mayo Clinic was electrostatically or covalently immobilized on the surface of the polymer films, and used to detect VEGF by electrochemical impedance spectroscopy (EIS). By fitting the impedance spectra to an equivalent circuit model, the leakage resistance was found to have a linear relationship with VEGF concentration.

Impedimetric biosensors for detecting VEGF based on PEDOT carboxylic acid/PEDOT copolymer

249th National Meeting (2015)

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