Series of nanoparticles (NPs) were derived using green colloidal chemistry. Natural products, such as green tea, coffee and vitamin C were used as both dispersing and reducing agents. The catechin content of the natural extracts was then analyzed by using high performance liquid chromatography to evaluate four major flavanol derivatives. These derivatives were used in production of NPs, such as silver and silver-titania. These NPs were formed through reduction oxidation (Redox) reaction, followed by the diffusion and aggregation. To determine crystalline phase, morphology and particle size and its distribution, the NPs were characterized using the state-of-the-art methods. It was also found that the NPs as disinfectants exhibited wide-spectra of activity toward Gram-negative/positive bacteria. The potency of bactericidal activities against Gram-negative bacteria was found to be 2.5 ppm within 2 hrs. The microbe cell membranes were damaged upon addition of the NPs. Some cytoplasmic membrane peeling may occur as the incubation time was increased and the leakage of reducing sugars and proteins was determined by EELS. The antioxidant quotient was evaluated by two standard assays, the Trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC). The antioxidant quotient (promotes cell survival) and ROS (promotes the cell death) have opposite contributions towards inhibition of microbes. The net contribution towards inactivation is the difference between these two factors.

Characterization and bioapplication of nanoscaled materials derived from green chemistry

249th National Meeting (2015)

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