The interactions between polyethylene glycol (PEG)-coated magnetic nanoparticles (MNPs) and human hemoglobin (Hb) were investigated using various spectroscopic techniques. UV-Vis spectrophotometry and dynamic light scattering (DLS) indicated strong binding between Hb and MNPs. Fluorescence quenching experiments were used to determine the binding constant (Ka), enthalpy changes (ΔHθ­­), entropy changes (ΔSθ­­), and free energy changes (ΔGθ). Results indicated that PEG-coated nanoparticles quenched hemoglobin fluorescence mainly by a static quenching mechanism. The binding constants (Ka) were determined as 1.12 ± 0.18 × 107 M-1, 0.95 ± 0.12 × 107 M-1, and 0.78 ± 0.16 × 107 M-1 at 297, 302, and 307 K, respectively. The changes of secondary structure of hemoglobin due to binding with nanoparticles were also investigated using circular dichroism (CD) spectroscopy.

Probing the interactions of polyethylene glycol-coated magnetic nanoparticles with human hemoglobin

249th National Meeting (2015)

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