Moderate consumption of red wine has been associated with reduced risk of heart disease and extended lifespan. These benefits are often attributed to trans-resveratrol, a polyphenol found in red grapes. Novel quantitative methods were developed for measuring trans-resveratrol in red wine samples using solid phase extraction (SPE) and solid phase microextraction (SPME). Each method employed an octadecylsilyl stationary phase. The extracts were analyzed by HPLC. Particular focus will be given to the SPME method, as these octadecylsilyl fibers are relatively new and have not been previously used for trans-resveratrol analysis. Additionally, it has been determined through preliminary testing that the SPME fibers can be used more often than the manufacturer’s recommendation. If proven true, the multiple use of these fibers can result in a significant cost reduction for the analysis. This new SPME method will be compared to the more conventional SPE method for ease of use, accuracy, and limit of detection.

Comparison of solid phase extraction and solid phase microextraction for the quantitative analysis of trans-resveratrol in red wine samples by HPLC

249th National Meeting (2015)

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