2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a toxic chemical formed through the manufacture of chlorinated phenols, primarily as a by-product of paper chlorination. This toxin is highly resistant to degradation and therefore easily accumulates in soil and groundwater. Previous research has shown that an amount lower than the concentration allowed in drinking water by the EPA is capable of decreasing estrogen levels. Estrogens lead to gene expression by binding to nuclear receptors. Expression of two target receptors, estrogen receptor beta (ERβ) and aryl hydrocarbon receptor (AHR), in luteinizing human granulosa cells were analyzed by quantitative real-time polymerase chain reaction (RT-qPCR), to explore where TCDD effects the expression of ERβ or AHR when cells were exposed to a concentrations of TCDD 1000 times lower than the maximum amount of toxin legally considered safe.

Effect of 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) on estrogen receptor beta and aryl hydrocarbon receptor using RT-qPCR

249th National Meeting (2015)

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Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on estrogen receptor beta and aryl hydrocarbon receptor using RT-qPCR