The parabens are antimicrobials used in cosmetics as a preservative that prevents fungal and microbial growth. However, the side effects of parabens include: endocrine disruptive effects, increased activity of estrogenic receptors, sensitization with broken skin, and adverse reproductive effects. Methylparaben is the most commonly used paraben in topical skin products. UVB radiation is known to increase the risk of skin cancer and is the most prevalent factor in skin cancer patients. In this study, M624 human melanoma cells were treated with methylparaben and UVB light. It is shown that expression of the apoptotic TNF- receptor is increased in the lipid rafts of the M624 cells treated with 5mM of methylparaben, but not in M624 cells treated with 5mM methylparaben and 25mJ/cm<sup>2</sup> UVB light. Expression of the Cytochrome C protein that is downstream in the TNF- pathway will also be analyzed via western blot after mitochondrial isolation in this study.

Lipid raft TNF-α pathway analysis of cytochrome C with<br />
methylparaben and UVB treatment

249th National Meeting (2015)

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Lipid raft TNF-α pathway analysis of cytochrome C with<br /> methylparaben and UVB treatment