We report here representative student data for a simple quantitative analysis of glucose using the enzyme glucose oxidase as coupled to a colorimetric reaction. A microplate reader was employed to rapidly determine absorbance values for multiple aliquots of a range of standard concentrations of glucose. A standard curve was then prepared and used to determine the concentration of an unknown glucose sample. In a separate exercise, students used a single-beam spectrophotometer to investigate enzymatic decomposition of glucose over time. Absorbance/time data were then evaluated using zero, first, and second order integrated rate equations to determine the order of reaction as well as the experimental rate constant. This experiment is most appropriate for use in an introductory (first or second year) quantitative analysis course, as it provides a correlation between biochemical catalysis and simple rate laws, as opposed to the more sophisticated Michaelis-Menten kinetic model utilized by biochemists

Quantitative analysis of glucose and kinetic study of glucose oxidase for use in an introductory quantitative analysis laboratory

249th National Meeting (2015)

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