The sandwich format biosensor is widely adopted for the detection and sensing of targets due to its flexible implementation of signal generation. By taking advantage of the easy modification of aptamer over antibody, which is not reducing its affinity to target, dual aptamers development is indispensable. In this study, we successfully screened dual aptamers specifically binding to one of the adipokines as a target protein by an immobilization–free SELEX method using graphene oxide (GO). GO-SELEX provides advantage in the increases exposure of the target’s surface thus give higher chances for ssDNA-target binding. The specificity and affinity of screened aptamers were analyzed by surface plasmon resonance (SPR) analysis. We further confirmed the binding of the dual aptamers at different sites on the target protein by quantum dot-labelled fluorescent imaging analysis. The dual aptamers produced was proved to enhance the detection of target more than 30 times using aptamer conjugated-AuNP based sandwich-type SPR method which then could be applied for aptasensor.

Ultrasensitive sandwich-type SPR aptasensor using dual aptamers developed by target immobilization-free SELEX

249th National Meeting (2015)

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