Metabolite X is essential for healthy metabolic status and the most of metabolite X we need can be synthesized from the external causes. Nevertheless, more than one billion people in the world are suffering from metabolite X insufficiency. Since the receptors of the metabolite X are found in almost every tissue in the human body, there is a high correlation between metabolite X and diverse diseases. It has been reported that there are increasing risks for rheumatoid arthritis, metabolic syndrome, cancers, cardiovascular disease and reduction in life expectancy, depending on the level of metabolite X deficiency. Therefore, in this study, for the prevention of metabolite X deficiency we developed a biosensor using aptamers which were successfully screened out by using the immobilization-free graphene oxide (GO) SELEX method. As a result, the aptamers found to show a great specificity to metabolite X, with the limit of detection of 1uM and 11nM of its Kd value. These metabolite X binding aptamers are expected to be widely used in the near future within a form of aptamer-based metabolite X diagnostic system. This could play a significant role in the fields of medical diagnostics with rapid, simple and accurate analytical method.

Development of highly sensitive aptamers to metabolite X for an aptamer-based diagnostic system

249th National Meeting (2015)

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