One of the possible structures for these ions is fructosyl sucrosyl-1,6-di-phosphate, with the two phosphate groups located at the two primary positions of the fructo-furanoside of the sucrose of the trisaccharide. This compound may be an effective lead for the inhibition of glycolysis, at elevated levels in cancer, at the point of conversion of fructose-1,6-bisphosphate to di-hydroxy acetone phosphate and glyceraldehyde-3-phosphate with fructose-1,6-bisphosphate aldolase. Also glycolytic aldolase A and C have been shown to bind to NF-L, neutrofilament L, mRNA that is a portion of mRNA of the total NF-L mRNA important in preventing neuro-degenerative disease. Aldolase is elevated in amyotropic lateral schlerosis (ALS) and over-expressed NF-L, in a mouse model, delays onset of ALS. Thus inhibitors, possibly the compound from ethanol extract from banana, of this enzyme may be of help in treating ALS. Alzheimers Disease aldolase is know to be present in AD patients and an antibody to it causes a lowering of AD symptoms in mice. Inhibition of this enzyme may be a pathway to the inhibition of AD symptoms in humans. Aldolase in Mycobacterium tuberculosi is known to be important in its metabolism. Inhibitors of its aldolase may be an effectiive treatment for this microbe. We have discovered a novel trisaccharide from an ethanol extract of banana fruit. We have done ICP-MS and ICP-MS/MS of the molecule in negative mode. We allowed a prepared ethanol extract of banana fruit to stand for 2 weeks or less before we did ICP-MS in the negative ion mode. The positive mode indicates the presence of sucrose, fructose and di-phospho fructosyl sucrose. The negative mode ICP-MS indicates intact di-phosphoryl fructosyl sucrose and its cleavage ions. We also present negative ion mode ICP-MS from a portion of the banana fruit extract treated diluted with ~1N ammonium hydroxide and to this solution sodium borohydride. This solution is allowed to stand for more than 1 day. After more than one day at these conditions the solution is diluted with ethanol and submitted for analysis by an ICP-MS instrument. We have evidence of hydride inserted fructosyl di-phospho fructosyl sucrose molecular ion, m/z 719.209 and molecular ion, m/2, 376.79. We will present their possible structure along with the accuracy of calculated versus found atomic mass units for the proposed ions for identity confirmation.

Structural characterization of a newly discovered trisaccharide in banana fruit ethanol extract

249th National Meeting (2015)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.