In China, gastric cancer is still the main cause of death in patients with malignant tumors. Cyclooxygenase-2 (COX-2) and B-cell lymphoma-2 (BCL-2) overexpression has recently been reported as a potential tumor initiator or promoter implicated in gastric carcinogenesis. In this study, we genotyped the COX-2 -765G&gt;C (rs20417) and BCL-2 -938C&gt;A polymorphisms in 118 gastric cancer patients and 120 healthy controls, using a method based on magnetic nanoparticles (MNPs) and dual-color fluorescence hybridization. The associations between COX-2 -765G&gt;C and BCL-2 -938C&gt;A polymorphisms and risk of gastric cancer have been investigated. Samples with COX-2 gene -765GC and -765CC genotypes were significantly higher than that with COX-2 gene -765GG genotype in gastric cancer group (OR=1.808, 95%CI: 1.050-3.113, P=0.033). Therefore, the carriers of C allele of COX-2 -765G/C polymorphism are more prone to gastric cancer in comparison with non-carriers. The gastric cancer individuals carrying BCL-2 938CC genotype have a 3.185-fold risk of gastric cancer than those carrying the AA genotype. In comparison with COX-2 -765GG and BCL-2 -938AA, risk of gastric cancer in COX-2 -765CC and BCL-2 -938CC, COX-2 -765GC and BCL-2 -938CC is significantly higher (OR=10, 95% CI: 1.280-78.117, P = 0.028; OR = 4.667, 95% CI: 1.137-19.154, P = 0.033). The genetic polymorphism of COX-2 -765G&gt;C and BCL-2 -938C&gt;A may jointly affect the onset of gastric cancer.

Genotyping of COX-2 and BCL-2 polymorphisms associated with gastric cancer susceptibility based on magnetic nanoparticles and dual-color fluorescence hybridization

249th National Meeting (2015)

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