Cellulosic materials exhibit many desirable characteristics, e.g. biodegradability and hydrophilicity, which make it an appealing substrate for various biomedical applications. Modification of cellulosic surfaces to create anti-fouling properties as well as to control protein adsorption is paramount to the functionality in these applications. Poly(ethylene glycol) or its derivatives incorporated in multifunctional polymers have been found to effectively prevent non-specific surface protein adsorption. In this work we present a method to prepare a low-fouling TEMPO-oxidized nanofibrillar cellulose (NFC) towards human immunoglobulin G (hIgG) utilizing block copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) . POEGMA-b-POEGMA copolymer adsorption on regenerated cellulose, NFC and TEMPO-oxidized NFC films was investigated with quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR). Atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) were applied to further verify that the adsorption of the block-copolymers was mainly electrostatically driven. The POEGMA-block-POEGMA copolymer adsorbed on TEMPO-NFC surfaces could be partially regenerated in low pH media and this effect was utilized to control bovine serum albumin (BSA) adsorption and desorption. The block-copolymers were observed to be good in preventing non-specific hIgG binding. The cationic PDMAEMA block of the copolymer functioned as an anchor to the anionic TEMPO-NFC surface while the POEGMA block provided surface passivating properties.<br/>

Modification of cellulose with PDMAEMA-block-POEGMA copolymers to control protein affinity for detection and separation

249th National Meeting (2015)

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