mRNA-lipoplexes are capable of inducing cytotoxic T cells (CTL) following subcutaneous immunization. Transfection of dendritic cells with mRNA-lipoplexes is accompanied by a strong induction of type I IFNs. Remarkably, these type I IFNs decrease the strength of the evoked response, as evidenced by an over tenfold increase in CTL numbers in IFNaR-/- mice. Although type I IFN have positive effects on the quality of the immune response following immunization with protein antigens, they negatively interfere with the strength of mRNA based vaccination by imposing an antiviral status upon the dendritic cell, resulting in inhibition of antigen translation and diminished antigen presentation.

mRNA based startegies for steering and amplifying the adaptive immune response

249th National Meeting (2015)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.