Rodenticides are used world-wide to control rodent populations in agricultural and urban settings. A sensitive method was developed to assess exposure of non-target avian species to seven rodenticides (warfarin, diphacinone, chlorophacinone, bromadiolone, brodifacoum, difethialone, and desmethyl bromethalin). The QuEChERS (quick, easy, cheap, effective, rugged and safe) methodology was modified for use with avian liver, kidney and carcass tissues. Homogenized tissues were fortified with surrogate standards and extracted with a water/acetonitrile solution followed by addition of excess NaCl. An aliquot of the extract was cleaned-up dispersive solid-phase extraction (dSPE) using PSA and C18 sorbents and magnesium sulfate. Extracts were analyzed by liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCI/MS/MS) operated in negative ion mode. Analyte recovery from quail tissues fortified with seven rodenticides was on average 103(±9) %. Detection limits were less than 10 ng/g for six of the seven rodenticides.

Determination of multiple rodenticides in avian tissues using a modified QuEChERS technique and LC-APCI/MS/MS detection

250th National Meeting (2015)

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