We have developed a highly sensitive fluorescent probe, BCu, for quantification of H2S releasing capacity in cell line model. MCF-7 cells in 96 well plate were incubated with a H2S probe BCu for 3 h, then treated with dietary organosulfides, which are biotransformed in the cells to produce H2S. The H2S releasing activity was quantified by fluorescent turning on degree of the probe. A dose response between organosulphur concentration and fluorescence intensity was applied to quantify the H2S releasing capacity of a given sample. We measured the the H2S releasing capacity of ten organosulphur rich fruits and vegetables (garlic, red onion, yellow onion, scallion, shallot, leek, spring onion, Chinese chives, durian, and stinky beans) and found that, stinky beans (a vegetable from Southeast Asia) exhibits extraordinary H2S donating capacity. The structure and H2S releasing activity of the unique cyclic polysulfides are discussed. Our research shed some light on the potential action mechanisms organosulfide rich foods in promotion of cardiovascular health.

Characterization of the activity of dietary organosulfides from vegetables as natural donors of hydrogen sulfide in cell line model

250th National Meeting (2015)

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