Anionic substances analyzed by reverse phase mode generally require pretreatment of the sample by derivatization or addition of ion pair reagent to the eluent. In this study, a multimode column (RP + anion exchange or HILIC), with polyvinyl alcohol packing material modified with quaternary ammonium functional groups was used under conditions without derivatization or ion pair reagent for the LC/MS analysis of various anionic substances. One example of this column applies to the analysis of haloacetic acids which are common undesirable disinfection byproducts (DBPs) generated during water purification. In the past, GC/MS was used as the standard method for haloacetic acid analysis; three haloacetic acids (monochloroacetic acid, dichloroacetic acid, and trichloroacetic acid) were simultaneously analyzed by LC/MS with the multimode column. The optimized eluent condition was 25 mM CH3COONH4 aq. (pH 9.2)/CH3CN = 50/50. The flow rate was 0.2 mL/min, and the column temperature was 30 °C. ESI-MS with SIM and MRM mode was used for detection. The mixed standard solution (2 ng/mL of each) was analyzed. All peak shapes were sharp with baseline separation, and the calibration curves showed high linearity. Tap water was analyzed without interference of detection. We will also introduce the applications of other substances (e.g. oxyhalides including perchloric acid, iodine ion, organic acids, allantoin, and phosphorylated saccharides). The multimode column is useful for the LC/MS analysis of various anionic substances using more simple conditions than previous methods.

LC/MS analysis of various anionic substances using polymer-based multimode column

250th National Meeting (2015)

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