Polymeric nanoparticles are currently being investigated as a method of drug delivery to multiple organ systems, including the lungs and respiratory passages. Recently, researchers have functionalized lipooligosaccharide (LOS) from the bacterium nontypeable <i>Haemophilus influenzae</i> (NTHi) onto polystyrene nanoparticles, successfully granting the nanoparticles the unique cellular invasion properties of NTHi. The present study aimed to optimize the concentration of LOS used to coat the particles in order to maximize the number of LOS molecules bound to the surface of 200 nm polystyrene nanoparticles. Isothermal titration calorimetry was used as the main quantification method, while dynamic light scattering and laser Doppler velocimetry were used to confirm and characterize the effects of LOS-nanoparticle binding. It was found that a titration concentration of 50 mg/mL LOS produced maximum LOS adsorption onto the surface of the nanoparticle. Since this is a slightly higher concentration than has been used in previous cell studies of LOS-coated particles, these results suggest that nanoparticle-cell binding might be improved by altering the concentration of LOS used during the coating process.

Characterization of lipooligosaccharide binding to polystyrene nanoparticles

251st National Meeting (2016)

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