We propose a redox responsive polymeric nanoparticle platform for biologics delivery. These stimuli responsive nanoparticles were synthesized using inverse emulsion methodology using a redox responsive polymeric crosslinker. Polymeric nanogels were synthesized In situ while sequestering biologic cargo such as plasmid DNA, siRNA, m RNA and proteins with high efficiency. The size of these nanoparticles are in the range of 100-200 nm and are non-toxic to the mammalian cell lines. Upon application of stimuli, the size of these particles decrease drastically as tested from dynamic light scattering experiments and also release the entrapped biologics confirmed through gel electrophoresis experiments. These evidences show complete disassembly of the nano aggregates upon application of redox trigger. These particles also possess surface functionalization feature which will be helpful in conjugation of ligands specific for cell surface receptors of interest. The cell uptake study of these nanoparticles was tested using confocal microscopy and the cells show good uptake of these nanoparticles into the cytosol.

Redox responsive nanogels for hydrophilics delivery

251st National Meeting (2016)

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