In this study, we describe the design of DNA-coated silica-iron oxide mesoporous nanocomposites for large protein (4 to 20 nm) co-delivery into Hela cells. This facile technique allows for a high yield production of uniform nanocomposites of 150 nm endowed with large pore size of 20 nm. The silica-iron oxide mesoporous nanocomposites annealed at 430 °C show superparamagnetism with saturation magnetization of 1.56 emu g <sup>−1</sup>,which gives the potential of biological application including hyperthermia. We used Histidine-monomeric teal fluorescent protein (His-mtfp), ferritin labeled with alexa fluorophore 647, and ferritin labeled with Histidine -monomeric teal fluorescent protein (His-mtfp), of respective sizes of 4 nm, 14 nm and 20 nm as a model proteins loaded into nanocomposite. In order to protect protein from premature degradation and various denaturing interactions with the biological environment. Double stranded DNA-capped iron oxide-silica nanocarriers of proteins were efficiently releasing simultaneously DNA and proteins under acidic pH or temperature stimuli, with sustained release over 70 h. Our system is expected to be a promising for large biomolecules delivery system.

Engineering large pore mesoporous silica nanoparticles for progene delivery application

251st National Meeting (2016)

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