I will present our work in using super-resolution single-molecule tracking to study transcription regulation in living cells. Binding and unbinding of transcription regulators on chromosome constitute a primary mechanism for gene regulation. While many cellular factors are known to regulate their binding, little is known on how cells can modulate their unbinding for regulation. I will describe that two metal-sensing transcription regulators show unusual concentration and chromosome-conformation dependent unbinding kinetics in bacterial cells, which provide novel mechanisms for facile switching between transcription activation and deactivation <i>in vivo</i> and in coordinating transcription regulation of resistance genes with the cell cycle.<br/>

Novel mechanisms of transcription regulation in living cells: A lesson from metalloregulators

251st National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.