Here we report the synthesis of a fluorogenic probe for labeling tyrosine residues under biologically relevant conditions. This triazabutadiene-based reagent is used to generate a coumarin diazonium species in moderately acidic conditions. The generated species forms an azo-bond with tyrosine residues: an event that is marked by a significant increase of fluorescence intensity. The utility of this reagent was demonstrated by fluorescence modification of a model protein.

Design and synthesis of triazabutadiene-based fluorogenic probe for tyrosine-specific labeling of proteins

251st National Meeting (2016)

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