Spruce xylan represents a versatile biopolymer for developing novel biomaterials. Silver-releasing hydrogels based on spruce xylan have a great potential for wound healing applications. Arabinoglucoronoxylan from Norway spruce was isolated, purified and characterized with nuclear magnetic resonance (NMR), infrared spectroscopy (FTIR) and size exclusion chromatography (SEC). Hydrogels were prepared by synthesis of a conjugate with tyramine. Conjugation was carried out by activation of carboxylic acid containing moieties (from glucuronic acid pending groups on the main xylan chain) with N-hydroxysuccinimide / 1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide (NHS / EDAC) and formation of amide bonds with tyramine. Gelation process was enabled by crosslinking the tyramine grafts with horseradish peroxidase enzyme (HRP) via addition of peroxide. Antibacterial properties were provided by <i>in situ</i> formation of silver nanoparticles (AgNPs) via incorporation of AgNO<sub>3</sub> during gelation and the subsequent reduction to silver nanoparticles.

Antibacterial hydrogels based on spruce xylan loaded with silver nanoparticles for biomedical applications

251st National Meeting (2016)

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